Fluorescence Molecular Imaging

Within optical imaging techniques, near-infrared fluorescence (NIRF) imaging is of particular interest for in vivo molecular imaging because at this wavelength range (650 – 900 nm) light can penetrate several centimeters into tissue and tissue autofluorescence is minimal. Other advantages of fluorescence imaging is the simultaneous detection of multiple probes with different spectral characteristics (multiplexing) and avoidance of ionizing radiation. This imaging technology is translatable to humans in the form of an endoscopic modality or a handheld device to perform image-guided surgery.

The aim of our fluorescence molecular imaging facility is the development of new molecular tracers based on the nanobody-technology for the visualization, characterization and quantification of molecular and cellular processes in vivo. The main steps along this process consist of (a) optimizing the conjugation between the nanobodies and NIRF fluorophores, (b) in-depth in vitro characterization of the tracers, (c) study of their in vivo pharmacokinetics and (d) in vivo evaluation of the tracer’s application in appropriate disease models. The final goal is to obtain fully characterized nanobody-based NIRF tracers that are ready for clinical translation.

  • Fluorescence Molecular Tomograph FMTTM2500 LX (Perkin Elmer)

The FMT is a 3D quantitative fluorescence imaging system dedicated to preclinical in vivo small-animal imaging. The FMT enables the detection of signals emitted in the near-infrared spectral window. It operates on 4 near-infrared channels (excitation/emission wavelength): 635/660 nm, 670/700 nm, 746/775 nm and 790/805 nm. Anatomical information can be provided after co-registration of the FMT datasets with computed tomography.

  • FluoBeamTM 800 (Fluoptics)

The FluoBeamTM is a real-time 2D optical imaging system that detects in vivo fluorescence in opened space. The laser of the FluoBeamTM 800 excites near-infrared fluorophores at 780 nm and a CCD camera detects fluorescence emission at >800 nm. The area of interest can be examined simultaneously under visible light, offering the possibility of intraoperative visualization of injected NIRF-labeled tracers.

  • Odyssey® Imager (Licor)
Odyssey Imager

The Odyssey® Imager offers a wide-range of in vitro and in vivo applications for near-infrared fluorescent detection (at 700 and 800 nm) including protein and nucleic acid gel visualization, ELISA and cell-based assays, tissue section imaging and small animal imaging.

  • EVOS FI (Life Technologies)

The EVOS FI microscope is equipped with a GFP, RFP, Cy5 and Cy7 Light Cube that enables the imaging of several fluorescent dyes, including near-infrared dyes, at cellular and sub-cellular level.


Dr. Sophie Hernot
0032 (0)2 477 49 91