Postdoctoral Fellows

Name:
Sophie Hernot

Degree:
Bio-Engineer - Medical Biotechnology
PhD in Medical Sciences
Professor

PhD Thesis:
Title: Nanobody-loaded and -coated micro bubbles: A tool for drug delivery and molecular imaging
Author: Sophie Hernot
Promotion Year: 2011
Promotor: Tony Lahoutte
Co-promotor: Nick Devoogdt & Bernard Cosyns
Flyer

  • S. Hernot, S. Unnikrishnan, T. Shevchenko, B. Cosyns, A. Broisat, J. Toczek, V. Caveliers, S. Muyldermans, T. Lahoutte, AL. Klibanov, N. Devoogdt. Nanobody-Coupled Microbubbles as Novel Molecular Tracer. J Control Release 158 (2012) 346-353.

  • S. Hernot, B. Cosyns, S. Droogmans, C. Garbar, P. Couck, C. Vanhove, V. Caveliers, G. Van Camp, A. Bossuyt, T. Lahoutte. Effect of High-Intensity Ultrasound Targeted Microbubble Destruction on Perfusion and Function of the Rat Heart Assessed by Pinhole Gated SPECT, Ultrasound Med Biol 36(1) (2010) 158-65.

  • S. Hernot, A.L. Klibanov, Microbubbles in Ultrasound-Triggered Drug and Gene Delivery, Adv Drug Deliv Rev 60(10) (2008) 1153-1166.



Affiliation:
In Vivo Cellular and Molecular Imaging Lab (ICMI), Vrije Universiteit Brussel (VUB)

Research Focus:
  • Near-infrared Fluorescence Imaging - Interventional Molecular Imaging
  • Cardiovascular Molecular Imaging - Atherosclerosis
  • Nanobody-based Probes

Contributions to science:
Design of nanobody-based fluorescent and bimodal tracers for use during image-guided surgery. The focus of the research is (a) evaluation of the effects of dyes and conjugation chemistry on the pharmacokinetics of nanobodies, and (b) preclinical validation of nanobody-based tracers for clinical translation.
  • P. Debie, M. Van Hoeij, N. Poortmans, N. Devoogdt, T. Lahoutte, S. Hernot. Image-guided surgery of intraperitoneally disseminated tumor lesions using near-infrared fluorescently-labeled nanobodies. In preparation (presented at EMIM Utrecht, The Netherlands, 2016)
  • P. Debie, J. Van Quathem, I. Hansen, G. Bala, S. Massa, N. Devoogdt, C. Xavier, S. Hernot. Effect of Dye and Conjugation Chemistry on Biodistribution Profile of Near-Infrared-Labeled Anti-HER2 Nanobodies (2017) Accepted in Molecular Pharmaceutics

Design of nanobody-based nuclear tracers enabling non-invasive characterization of atherosclerotic plaques. The research encompasses the development and preclinical in vivo validation of nanobodies targeting state-of-the-art biomarkers that are involved in the triggering of acute events. An important aspect is evaluation of different radiochemistries enabling future translation in humans and application in clinical routine.
  • G. Bala, H. Baudhouin, I. Remory, K. Gillis, P. Debie, A. Krasniqi, T. Lahoutte, G. Raes, N. Devoogdt, B. Cosyns, S. Hernot. Evaluation of Macrophage Mannose-Specific nanobodies for Imaging of Atherosclerotic Lesions. Submitted to Molecular Imaging & Biology (presented at TOPIM, les Houches, France, 2016)

  • G. Bala, A. Blykers, C. Xavier, B. Descamps, A. Broisat, C. Ghezzi, G. Van Camp, V. Caveliers, C. Vanhove, T. Lahoutte, S. Droogmans, B. Cosyns, N. Devoogdt, S. Hernot. Targeting of Vascular Cell Adhesion Molecule-1 by 18F-labeled nanobodies for PET/CT imaging of inflamed atherosclerotic plaques. (2016) Eur Heart J – Cardiovasc Imaging doi:10.1093/ehjci/jev346.

  • A. Broisat*, S. Hernot*, J. Toczek, J. De Vos, U. Wernery, L. Riou, S. Martin, M. Ahmadi, N. Thielens, V. Caveliers, S. Muyldermans, T. Lahoutte, D. Fagret, C. Ghezzi*, N. Devoogdt*. Nanobodies Targeting Mouse/Human VCAM1 for Nuclear Imaging of Atherosclerotic Lesions. *Authors contributed equally. Circulation Research 110(7) (2012)

Implementation of non-invasive (molecular) imaging techniques in a variety of research domains out of my direct field of interest, aiming to better understand biological processes in an in vivo situation (in compliance with the 3R principle, reduction, refinement and replacement).
  • E. Oyen, C. Martin, V. Caveliers, A. Madder, B. Van Mele, R. Hoogenboom, S. Hernot, S. Ballet. Biostability of and controlled cargo release from injectable amphipathic peptide hydrogels: An in vivo imaging study (2017) Accepted in Biomacromolecules

  • M. Morais, M. Campello, C. Xavier, J. Heemskerk, J. Correia, T. Lahoutte, V. Caveliers, S. Hernot, I. Santos. Radiolabeled Mannosylated Dextran Derivatives bearing a NIR-fluorophore for Sentinel Lymph Node Imaging. Bioconjug Chem 25(11) (2014) 1963-70. IF 4.513

  • J. Wang, A. Hendrix, S. Hernot, M. Lemaire, E. De Bruyne, E. Van Valckenborgh, T. Lahoutte, O. De Wever, K. Vanderkercken, E. Menu. The role of bone marrow stromal cell-derived exosomes as communicators in drug resistance in multiple myeloma cells. Blood 124(4) (2014)

  • A. Ceulemans, S. Hernot, T. Zgavc, V. Caveliers, S. Hachimi-Idrissi, S. Sarre, T. Lahoutte, Y. Michotte. Serial Semi-Quantitative Imaging of the Brain Damage using Micro-SPECT/Micro-CT after an Endothelin-1 Induced Transient Focal Cerebral Ischemia in Rats, J Nucl Med 52(12) (2011) 1987-92. IF 6.381


Complete List of Published Work:

http://www.ncbi.nlm.nih.gov/pubmed/?term=hernot+sophie

Address:

Vrije Universiteit Brussel
Department BEFY, Labo ICMI, Building K
Laarbeeklaan 103
1090 Jette (Brussels)
Belgium

Telephone:
Labo ICMI: 0032 (0)2 477 4991
GSM: 0032 (0)479 39 96 19

Email:
sophie.hernot@gmail.com